TABLE 1.

Quantitation of parasite loads in BALB/c mice infected i.p. with WT or Δsrs9 tachyzoites

Strain and area of infectionParasite load on daya:
0591418222835
Total ventral
    WT363 ± 287,813 ± 5,66523,813 ± 27,1621,518 ± 764543 ± 116438 ± 57417 ± 71347 ± 214
    Δsrs9350 ± 327,623 ± 2,45020,598 ± 17,482958 ± 401881 ± 262476 ± 97493 ± 47436 ± 140
Neck
    WT48 ± 560 ± 7254 ± 110110 ± 2351 ± 747 ± 1146 ± 1051 ± 7
    Δsrs950 ± 355 ± 8296 ± 8585 ± 2466 ± 1540 ± 948 ± 553 ± 9
Chest
    WT92 ± 5164 ± 14402 ± 296237 ± 99105 ± 1699 ± 889 ± 390 ± 7
    Δsrs993 ± 4205 ± 33435 ± 102192 ± 96141 ± 2998 ± 1187 ± 589 ± 4
Abdomen
    WT262 ± 338,560 ± 4,16023,254 ± 26,7171,160 ± 807364 ± 79304 ± 56264 ± 38250 ± 73
    Δsrs9279 ± 337,350 ± 2,41319,940 ± 17,354719 ± 356658 ± 184333 ± 84328 ± 39281 ± 106
Total dorsal
    WT296 ± 16NDND601 ± 253343 ± 50354 ± 36293 ± 24294 ± 40
    Δsrs9298 ± 15NDND639 ± 130740 ± 115438 ± 20281 ± 11297 ± 23
Brain
    WT68 ± 8NDND178 ± 5372 ± 1267 ± 773 ± 974 ± 4
    Δsrs970 ± 7NDND128 ± 50123 ± 3688 ± 2174 ± 472 ± 5
  • a Parasite loads are presented as photon flux detected by BLI in the regions of interest defined in Fig. 2B. Four mice per group were infected with the WT or Δsrs9 strain (500 tachyzoites i.p.). Mean levels of flux (103 photons/s/cm2/sr) ± standard deviations are shown. Notable differences are indicated in boldface and include the chest signals on day 5 (P = 0.07); total ventral signals (P = 0.06), abdominal signals (P = 0.03), total dorsal signals (P = 0.004), and brain signals (P = 0.04) on day 18; and total dorsal signals (P = 0.01) on day 22. ND, not determined, as brain signals do not appear until >10 days after infection.