TABLE 1

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsReference or source
E. coli strains
    NEB5αfhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17New England Biolabs
    BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Invitrogen
    YM1pYM1 in BL21(DE3)This study
    YM2pYM2 in BL21(DE3)This study
    YM3pYM3 in BL21(DE3)This study
    YM4pYM4 in BL21(DE3)This study
    YM5pYM5 in BL21(DE3)This study
    YM6pYM6 in BL21(DE3)This study
    YM7pYM7 in BL21(DE3)This study
    YM8pYM8 in BL21(DE3)This study
    YM9pYM9 in BL21(DE3)This study
    YM10pYM10 in BL21(DE3)This study
    YM11pYM11 in BL21(DE3)This study
Plasmids
    pJK654pJAK16 containing full-length apiA14
    pKD3Plasmid containing dspB and the aae C terminus for cell surface display of DspB28
    pET29a+Cloning vector; kanamycin resistantInvitrogen
    pYM1pET29a+ containing full-length apiA; designated ApiA in the textThis study
    pYM2pET29a+ containing truncated apiA with amino acids 284–295 deleted; designated the ApiAΔ284-295 variant in the textThis study
    pYM3pET29a+ containing truncated apiA with amino acids 186–217 deleted; designated the ApiAΔ186-217 variant in the textThis study
    pYM4pET29a+ containing truncated apiA with amino acids 186–240 deleted; designated the ApiAΔ186-240 variant in the textThis study
    pYM5pET29a+ containing truncated apiA with amino acids 186–229 deleted; designated the ApiAΔ186-229 variant in the textThis study
    pYM6pET29a+ containing truncated apiA with amino acids 28–185 deleted; designated the ApiAΔ28-185 variant in the textThis study
    pYM7pET29b+ containing recombinant truncated apiA with amino acids 218–295 deleted fused to the aae C terminus; designated the ApiAΔ218-295+Aae-Cter variant in the textThis study
    pYM8pET29b+ containing recombinant truncated apiA with amino acids 186–295 deleted fused to the aae C terminus; designated the ApiAΔ186-295+Aae-Cter variant in the textThis study
    pYM9pET29b+ containing recombinant truncated apiA with amino acids 28–185 and 218–295 deleted fused to the aae C terminus; designated the ApiAΔ28-185+Aae-Cter variant in the textThis study
    pYM10pET29b+ containing recombinant truncated apiA with amino acids 37–101 and 218–295 deleted fused to the aae C terminus; designated the ApiAΔ37-101+Aae-Cter variant in the textThis study
    pYM11pET29b+ containing recombinant truncated apiA with amino acids 46–90 and 218–295 deleted fused to the aae C terminus; designated the ApiAΔ46-90+Aae-Cter variant in the textThis study