TABLE 3.

Neutralizing activities of the immune seraa

ImmunogenMouse serumSerum dilutionb
NaïvePooledNP
BPZE1PooledNP
EV71Pooled1/128
BPSQ5M11/32
M21/2
M3NP
M41/16
M51/32
M61/32
BPSY13.1M1NP
M2NP
M3NP
M4NP
M51/16
M6NP
  • a Twofold serial dilutions of the sera from each group were incubated with EV71 before infection of RD cells, and the CPE were observed 48 h later. Sera from individual mice belonging to the naïve, BPZE1, and EV71 groups were pooled, while serum from each BPSY13.1- and BPSQ5-immunized mouse was tested individually.

  • b Highest dilution with which total protection was observed. NP, no protection.