TABLE 1.

TB lesions and distribution/accumulation of Vγ2Vδ2 T cells in organs/tissues of four rhesus monkeys with the severe form of tuberculosisa

Tissueb% Vγ2Vδ2 T cellscM. tuberculosis CFUcM. tuberculosis mRNA levelcGross and microscopic TB lesions
BM2.5 ± 0.71.0 ± 0.3ND
BLN11.9 ± 4.2262 ± 10216,046 ± 3,156Gross granulomas
Mesenteric LN7.3 ± 2.3232 ± 89No apparent TB lesions
Spleen14.8 ± 5.3ND101 ± 26Focal mild (micro) granulomas (<2 mm) seen in two monkeys
Thymus3.0 ± 1.2ND235 ± 155
Blood1.8 ± 0.316.3 ± 13.333 ± 23
Lung23.0 ± 5.1535.5 ± 163.57,122 ± 4,795Numerous gross granulomas
Liver13.8 ± 2.2ND430 ± 312One monkey showed two small granulomas at gross pathological evaluation; microscopic analysis showed 1 to 10 mild granulomas (<2 mm) in two monkeys and no lesions in the other two monkeys
Kidney32.1 ± 13.4ND121 ± 80No detectable TB lesions
Intestine25.3 ± 14.3ND242 ± 164No detectable TB lesions
  • a Three cynomolgus monkeys were euthanized due to severe TB 11 weeks after pulmonary M. tuberculosis infection. Necropsies showed that there were numerous microgranulomas in the lungs and bronchial lymph nodes, with or without focal microgranulomas in the liver, spleen, kidneys, or intestinal mucosa.

  • b BM, bone marrow; BLN, bronchial lymph nodes; LN, lymph nodes.

  • c Data are means ± standard deviations. ND, not done.