TABLE 4

Strains and plasmids used in this study

Strain or plasmid (identifier)Relevant genotype or commentsSource and/or reference
Strains
    S. Typhimurium
        14028 (ICC797)WT64
        14028 (ICC1765)ΔhilA::lacZ KanrThis study
        14028 (ICC1764)ΔhilD::lacZ KanrThis study
    S. Typhi
        Ty2 (ICC1500)WTG. Dougan
        Ty2 (ICC1630)ΔhilA::lacZ KanrThis study
        Ty2 (ICC1762)ΔhilD::lacZ KanrThis study
        Ty2 (ICC1556)ΔinvA Kanr64
        Ty2 (ICC1756)Δpat KanrThis study
    CT18 (ICC1502)WTG. Dougan
    129-0238 (ICC1503)WT, H58 isolateG. Dougan (21)
    ERL12148 (ICC1504)WT, H58 isolateG. Dougan (21)
Plasmids
    pKD4 (pICC893)Kanamycin cassette template plasmid63
    p3138 (pICC2515)LacZ and kanamycin cassette template plasmid41
    pKD46 (pICC1298)Lambda red recombinase plasmid63
    pWSK29-Spec E.V. (pICC2489)Empty vector, spectinomycinr64
    pWSK29-Spec HilD-4HA Ty2S. Typhi Ty2 HilD-4HA, constitutive promoterThis study
    pWSK29-Spec HilD 4HA TmS. Typhimurium 14028 HilD-4HA, constitutive promoterThis study