Table 1.

Effects of immunization or infection on hsp65-reactive T-cell frequencies in spleena

TreatmentFrequency of T-cell response to Hsp65
CD4CD8
rhsp651:3,5501:48,000
hsp65 DNA1:1,8001:1,480
M. tuberculosis 1:4,9001:5,500
Saline (control)<1:100,000<1:100,000
Vector DNA (control)<1:100,000<1:100,000
  • a Splenocyte subpopulations were purified by combined positive and negative selection 2 weeks after completion of immunization or 4 weeks after infection; the frequencies of hsp65-reactive cells were then assessed by limiting dilution analysis with J774-hsp65 as antigen-presenting cells.