TABLE 1.

Primer sequences used to amplify TFSS genes

Gene nameForward primerReverse primera
VirB95′-ATGAATTTCTATAAAAACTTGCTTGCGTGCTCGGC-3′5′-TTTGTCGTCGTCGTCTTTATAGTCAAGCACCGTATTCACTACTTCGAC-3′
VirB105′-TTGAGTTTAGGGATGTCAGACGAAACCAAGG-3′5′-TTTGTCGTCGTCGTCTTTATAGTCCCTACGCACCGCCTCCCTAG-3′
CTP5′-ATGAAAAAGGCTTTCATGGTTTGTGCTGTAGCGC-3′5′-TTTGTCGTCGTCGTCTTTATAGTCCCCACGTCCCCTTCTG-3′
  • a Reverse primers contain the 30-nucleotide FLAG tag sequence, which is boldfaced.