TABLE 4.

B. quintana proteins found to be immunoreactive with sera from patients infected with Bartonella

SpotGeneProtein description% of patients reactiveaVol of spotb
MeanMedianRange
OMP
    VompB vompB Variable outer membrane protein B3326.020.45.6-97.1
    VompA vompA Variable outer membrane protein A2931.522.36.0-120.2
    JB33 ppi Peptidyl-prolyl cis-trans-isomerase298.47.52.2-17.8
    JB114 hbpE Hemin-binding protein E245.23.81.3-12.5
Non-OMP
    JB16 sucB Dihydrolipoamide succinyltransferase7634.021.23.1-146.8
    JB17 sucB Dihydrolipoamide succinyltransferase7617.69.90.5-89.4
    JB15 sucB Dihydrolipoamide succinyltransferase5220.39.82.4-101.5
    JB12 guaB Inosine-5′-monophosphate dehydrogenase5211.87.52.3-51.2
    JB38 tuf1 Elongation factor tu487.96.13.1-18.1
    JB51 mopA Chaperonin protein GroEL4819.313.92.3-62.5
    JB52 mopA Chaperonin protein GroEL4824.119.81.9-104.0
    JB74 mopA Chaperonin protein GroEL489.06.51.7-29.0
    JB14 atpD ATP synthase beta chain4315.68.83.7-50.0
    JB37 tuf1 Elongation factor tu4310.47.51.7-47.4
    JB79 tuf1 Elongation factor tu4313.810.62.3-69.3
    JB10 rplY 50S ribosomal protein L253831.522.94.6-87.9
    JB11 sucD Succinyl-coenzyme A synthetase alpha chain388.57.34.1-16.2
    JB19 rpsA 30S ribosomal protein s1385.85.50.7-11.3
    JB65 ppdK Pyruvate phosphate kinase384.43.00.7-15.2
    JB9Hypothetical protein3315.210.14.5-44.6
    JB21 rpsA 30S ribosomal protein s1339.55.21.2-53.5
    JB47 atpD ATP synthase beta chain3310.67.54.3-30.2
    JB55 dnaK Heat shock protein 70 DnaK3328.227.26.6-72.7
    JB109 mopA Chaperonin protein GroEL3351.839.23.5-217.7
    JB25 ibpA2 Small heat shock protein298.36.82.3-15.1
    JB29 purC Phosphoribosylaminoimidazole-succinocarboxamide synthase294.73.81.9-8.7
    JB45 atpA ATP synthase alpha chain295.63.41.6-13.3
    JB56 ftsZ Cell division protein FtsZ homolog295.74.61.5-13.8
    JB57 typA GTP-binding protein TypA294.34.02.7-6.9
    JB63 pnp Polyribonucleotide nucleotidyltransferase294.14.31.1-7.1
    JB18 rho Transcription terminator factor rho246.04.50.3-15.5
    JB44 atpA ATP synthase alpha chain245.03.32.5-11.0
    JB50 tig Trigger factor2416.76.41.9-57.9
    JB73 guaB Inosine-5′-monophosphate dehydrogenase247.23.42.7-20.1
    JB121 mopA Chaperonin protein GroEL244.02.71.5-7.2
    JB133ABC transporter, ATP-binding protein244.13.30.8-8.9
  • a Percentage of patient sera found to have immunoreactivity to the protein of the total number of patients (n = 21).

  • b The volume of a spot was determined by dividing the pixel intensity by the area of the spot (2D Evolution Analysis software; Nonlinear Dynamics, Durham, NC).