TABLE 1

Calculation of LD50 for zebrafish

Dose of challenge (no. of CFU/0.02 ml)No. of dead animals/total no. tested after infection with:
Wild typeΔcas9 mutantcas9 mutant
2 × 10614/1414/1414/14
2 × 10514/1412/1414/14
2 × 10414/1411/1414/14
2 × 10310/148/1412/14
2 × 1026/144/145/14
2 × 1013/140/141/14