TABLE 3

Summary of presence of recombinant spores of the B. subtilis tasA sinR lux TasA-mCherry strain in the feces of dogs after the first and second oral applications

DPAaPresence of recombinant spores in the indicated dog afterb:
First oral applicationSecond oral application
45Placebo-treated dog45Placebo-treated dog
1
2++
3
4
5
6
  • a DPA, days postapplication.

  • b Dogs 4 and 5 were treated with 5 × 1010 CFU spores/dose of the B. subtilis tasA sinR lux TasA-mCherry strain diluted in milk, and the placebo-treated dog was treated with milk only. Anal swab samples samples were then collected and tested. The samples were considered positive (+) when they were resistant to heat treatment (80°C for 20 min) and displayed luminescence after germination, as inspected with an IVIS camera.