Table 3.

Assessment of H. pylori presence in gastric biopsy specimens in immunized micea

RUT resultsNo. of H. pyloribacteria/10 crypts
phoPc tac-ureABcT7-ureAB
Positive39 ± 25 (n = 9)b 28 ± 17 (n = 8)23 ± 26c (n = 2)
Negative0 (n = 0)0 (n = 0)<1 ± 1 (n = 7)d
  • a One half of the stomach of each mouse immunized with S. typhimurium phoPc ,S. typhimurium phoPc tac-ureAB, or S. typhimurium phoPc cT7-ureAB was subjected to the RUT, and the other half was processed for microscopic analysis as described in Materials and Methods.

  • b n = number of mice.

  • c P = 0.006 compared to value forphoPc -immunized mice; P = 0.0008 compared to value for phoPc tac-ureAB-immunized mice (by Wilcoxon rank sum test).

  • d Six animals were found free of bacteria by microscopy, but one presented 4 H. pylori bacteria per 10 crypts.