TABLE 2.

Competitive colonization between sugar mutants and wild-type E. coli strains

SugarMutation(s)Log10 difference (CFU/g ± SEM) for E. coli EDL933 populationsa on:Log10 difference (CFU/g ± SEM) for E. coli MG1655 populationsa on:
Day 1Day 9Day 1Day 9
ArabinosearaBAD0.4 ± 0.21.4 ± 0.30.1 ± 0.12.2 ± 0.3
FucosefucAO0.4 ± 0.42.8 ± 0.51.1 ± 0.21.8 ± 0.3
GalactosegalK0.9 ± 0.52.9 ± 0.40.4 ± 0.10.8 ± 0.2
GluconatebgntK (idnK)0.3 ± 0.30.6 ± 0.50.6 ± 0.11.4 ± 0.3
HexuronatescuxaC0.1 ± 0.11.2 ± 0.20.0 ± 0.40.0 ± 0.2
GalacturonateuxaB0.2 ± 0.20.8 ± 0.30.4 ± 0.30.8 ± 0.3
MannosemanA0.6 ± 0.22.2 ± 0.90.3 ± 0.50.1 ± 0.2
NAGnagE0.3 ± 0.31.6 ± 0.41.2 ± 0.22.2 ± 0.3
GlucosaminemanXYZ0.6 ± 0.60.5 ± 0.3NDdND
GalNAc and galactosamineagaWEFA0.6 ± 0.50.7 ± 0.4Pathway missingePathway missinge
NANAnanAT0.7 ± 0.2−0.6 ± 0.21.2 ± 0.33.3 ± 0.7
RiboserbsK0.2 ± 0.31.1 ± 0.30.0 ± 0.30.3 ± 0.2
  • a Each mouse was fed 105 CFU of a mutant strain and its wild-type parent. Values shown are the log10 differences in populations between the mutant and the wild types; i.e., the log10 CFU/g of feces for the mutant was subtracted from the log10 CFU/g of feces for the wild type. The average log10 differences ± standard error of the mean (SEM) of day 1 and day 9 data from at least six mice are shown. Colonization differences that exceeded 1 log and are statistically significant (Student's t test, P < 0.05) are shown in bold.

  • b E. coli EDL933 is deleted for idnK, which encodes the secondary gluconate kinase.

  • c The uxaC mutation blocks catabolism of both hexuronates, glucuronate, and galacturonate.

  • d ND, not determined.

  • e E. coli MG1655 is deleted for agaWEFA. E. coli EDL933 and MG1655, carrying mutations for the catabolism of aspartate (aspA), galactitol (gatABC), glycerol (glpK), lactose (lacZ), and mannitol (mtlA), did not have colonization defects (data not shown).