TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or phenotypeSource or reference
MG1655Wild type (CGSC no. 7740)aCGSC
MG1655 StrrSpontaneous Strr36
MG1655 Strr NalrSpontaneous Nalr36
EDL933Wild-type O157:H7Alison O'Brien
EDL933 StrrSpontaneous Strr35
EDL933 Strr NalrSpontaneous Nalr35
Derivatives of strain MG1655 Strr
    araBADbΔ(araB-araD)::catThis study
    aspAΔaspA::cat6
    fucAOΔ(fucA-fucO)::kan6
    galKΔgalK::catThis study
    gatABCΔ(gatA-gatC)::catThis study
    glpKΔglpK::kan6
    gntK idnKΔgntK ΔidnK::cat30
    lacZΔlacZ::catThis study
    manAΔmanA::catThis study
    mtlAΔmtlA::catThis study
    nagEΔnagE::kan6
    nanATΔ(nanA-nanT)::kan6
    rbsKΔrbsK::kan6
    srlAEBΔ(srlA-srlB)::catThis study
    uxaBΔuxaB::kan6
    uxaCΔuxaC::kan6
Multiple-mutation derivatives of strain MG1655 Strrc
    fucAO rbsKThis study
    fucAO rbsK manAThis study
    fucAO rbsK manA araBADThis study
    fucAO rbsK manA araBAD nagEThis study
    fucAO rbsK manA araBAD nagE galKThis study
Derivatives of strain EDL933 Strr
    agaWEFAΔ(agaW-agaA)::catThis study
    araBADΔ(araB-araD)::catThis study
    aspAΔaspA::catThis study
    fucAOΔ(fucA-fucO)::kanThis study
    galKΔgalK::catThis study
    gatABCΔ(gatA-gatC)::catThis study
    glpKΔglpK::catThis study
    gntKΔgntK::catThis study
    lacZΔlacZ::catThis study
    manAΔmanA::catThis study
    manXYZΔ(manX-manZ)::catThis study
    mtlAΔmtlA::catThis study
    nagEΔnagE::catThis study
    nanATΔ(nanA-nanT)::catThis study
    rbsKΔrbsK::catThis study
    uxaBΔuxaB::catThis study
    uxaCΔuxaC::catThis study
Multiple-mutation derivatives of strain EDL933 Strrc
    fucAO rbsKThis study
    fucAO rbsK manAThis study
    fucAO rbsK manA araBADThis study
    fucAO rbsK manA araBAD nagEThis study
    fucAO rbsK manA araBAD nagE galKThis study
Plasmids
    pKD3bla cat11
    pKD4bla kan11
    pKD46Ts Apr ParaC-λ-red recombinase11
    pCP20Ts Apr FLP recombinase11
  • a CGSC, E. coli Genetic Stock Culture Collection, Yale University.

  • b For simplicity, strains are named in the text by strain and mutation(s).

  • c See Materials and Methods for details on multiple-mutation constructions; genotypes are identical to those listed in this table for single mutations.