TABLE 2.

Peptides used to generate antibodies that reacted with the peptide in an ELISA

PeptideLocation of peptide (aa)dSequence
OMP1-P1a151-160CTKKYKDNPERAYR
OMP1-P2a70-79CKEKQQGGTAK
OMP1-P3b279-287CTESPKGSQG
OMP2-P1a7-19CEQKRGRCESAR
OMP3-P1a35-50CITRHPSPTYHHSSPHR
OMP3-P2c141-155CATIYNHPMLSSQPHK
OMP3-P3c17-26CQCHHLCTTNT
OMP4-P1b176-190CSDTIESELFQHTGGD
OMP5-P1a188-100CQGGLSIDSSTSTA
OMP5-P345-53CGHKGAGTRR
OMP5-P2a224-245CSSEDRLAAAK
OMP6-P121-30CGTGSSAAEAF
OMP6-P2a131-139CGRHWKQGNS
OMP7-P1b198-209CDLKHVGASSVD
OMP8-P1b172-185CALPEHRDFLSLEDA
OMP9-P1b207-220CGTTREDALAATQIV
OMP10-P1192-200CEEGKRLGNL
OMP10-P2268-276CSDKDEARRA
OMP10-P3182-190CSTNAGDGKS
OMP11-P1b149-168CHDEGVVGDLYASE
OMP12-P1149-158CNIALVRAQT
OMP12-P26-16CRATKKGSISVR
OMP12-P339-48CRKFRSQGRAY
OMP13-P1199-210CNAAGAGSSAGQQ
OMP13-P3a317-325CGASSRTRDD
OMP14-P1a239-249CQNTQESKRDEA
OMP14-P2a378-388CLGKTKEKVSAS
OMP14-P3a36-46CASSHGMNGRED
  • a Peptides that elicited antibodies that reacted with the recombinant protein.

  • b Peptides that elicited antibodies that reacted with the recombinant protein and A. marginale proteins in erythrocytes and/or IDE8 cells.

  • c Peptides that elicited antisera that did not react with A. marginale-infected erythrocytes and/or IDE8 cells but were not tested against the recombinant protein. The remaining peptides elicited antisera that reacted with the peptide in an ELISA but did not react with recombinant proteins.

  • d aa, amino acids.