TABLE 1.

Bacterial Strains and plasmids used

Strain or plasmidRelevant characteristic(s)Reference or source
Y. pestis strains
    Kimberley53Virulent strain18
    Kimberley53Δp70/Δp10Spontaneously pPCP1- and pCD1-cured Kimberley53 strain18
    EV76pgm (Girard's strain)1
    EV76ΔyopJEV76 with knockout in yopJ gene; KanrThis study
    EV76ΔyopJ+yopPEV76ΔyopJ carrying pyopPThis study
    EV76ΔyopJ+yopJEV76ΔyopJ carrying pyopJThis study
Y. enterocolitica strains
    Y. entero.Virulent strain; WA O:8ATCC 27729a
    Y. enteroΔp70Spontaneously pYV-cured WA O:827
Plasmids
    pGFPuvlac-controlled gfp gene on pUC vector; AmprCLONTECH, USA
    pyopPpGFPuv in which the gfp gene is replaced by the Y. enterocolitica O:8 yopP geneThis study
    pyopJpGFPuv in which the gfp gene is replaced by the Y. pestis Kimberley53 yopJ geneThis study
    pKOBEG:sacBCarries the λ phage red operon and sacB gene; Cmr14
  • a See reference 27.