TABLE 4.

Correlation between CD-mucin binding inhibition titers and antibody titers

Wk 6 antiserum	CD dose (μg)	Adjuvant	Titer
Wk 6 antiserum	CD dose (μg)	Adjuvant	CD-mucin binding inhibition^a	Anti-CD	Anti-O35E whole cell
Anti-nCD	5	MPL/alum	650	284,657	34,588
Anti-rCD	5	MPL/alum	664	247,008	30,012
Anti-rCD	20	MPL/alum	1,556	3,049,725^b	240,860^b
Anti-rCD	20	Alum	2,157	4,448,261^b	406,666^b
		QS21	4,211	7,540,524^b	1,411,385^b
Anti-rCopB	20	MPL/alum	<50	<50	100,000
Anti-UspA	5	MPL/alum	<50	<50	<50
Anti-CRM197	5	MPL/alum	<50	<50	<50
Anti-E. coli lysate^c	5	MPL/alum	<50	<50	<50
Preimmune			<50	<50	<50

↵a The inhibition titer is the dilution of serum at which a 50% reduction of CD-mucin binding was observed.
↵b Mice were immunized with 20 μg of rCD for the murine pulmonary clearance studies.
↵c Antiserum to an induced E. coli BL21(DE3)/pRSET/Kan^r cell lysate.