TABLE 4.

Correlation between CD-mucin binding inhibition titers and antibody titers

Wk 6 antiserumCD dose (μg)AdjuvantTiter
CD-mucin binding inhibitionaAnti-CDAnti-O35E whole cell
Anti-nCD5MPL/alum650284,65734,588
Anti-rCD5MPL/alum664247,00830,012
Anti-rCD20MPL/alum1,5563,049,725b240,860b
Anti-rCD20Alum2,1574,448,261b406,666b
QS214,2117,540,524b1,411,385b
Anti-rCopB20MPL/alum<50<50100,000
Anti-UspA5MPL/alum<50<50<50
Anti-CRM1975MPL/alum<50<50<50
Anti-E. coli lysatec5MPL/alum<50<50<50
Preimmune<50<50<50
  • a The inhibition titer is the dilution of serum at which a 50% reduction of CD-mucin binding was observed.

  • b Mice were immunized with 20 μg of rCD for the murine pulmonary clearance studies.

  • c Antiserum to an induced E. coli BL21(DE3)/pRSET/Kanr cell lysate.