TABLE 1.

CD-specific antibody response elicited by either nCD or rCD in micea

Mouse serumAnti-CD antibody titer elicited by CD (μg)b
nCD (1)nCD (5)rCD (5)
Preimmune<50<50<50
Wk 419,22293,11089,515
Wk 658,451284,657247,008
  • a Mice were immunized with either nCD or rCD with MPL plus alum as adjuvant.

  • b Anti-CD antibody titers were the reciprocal of the serum dilution with an absorbance at 405 nm of 0.1 extrapolated from a linear plot of the logarithm of absorbance versus the logarithm of serum dilution. A negative-control antiserum from mice immunized with 5 μg of an induced E. coli BL21(DE3)/pRSET/Kanr cell lysate exhibited a titer of <50.