TABLE 1.

F. tularensis LVS genes essential for lung infection identified by STM

Function group and mutant IDaORF in LVSbORF in Schu S4cGenedPredicted functiondNo. of independent mutantseIn vitro GIf
Cell surface structures and membrane proteins
    JS4512FTL_0009FTT1747Outer membrane protein10.70
    JS3217FTL_0073FTT1676Membrane protein>50.89
    JS2802FTL_0421FTT0901tul4Lipoprotein, T-cell-stimulating antigen20.89
    JS4596FTL_0540FTT1568clpxBLipid A-disaccharide synthase11.00
    JS1430FTL_0589FTT1525cHypothetical membrane protein20.92
    JS3023FTL_0592FTT1464cwbtAdTDP-glucose 4,6-dehydratase, LPS modification10.74
    JS2516FTL_0597FTT1459cwbtFNAD-dependent epimerase, LPS modification20.70
    JS4788FTL_0645FTT1416cLipoprotein20.81
    JS2294FTL_1096FTT1103Lipoprotein20.78
    JS1965FTL_1134NAMembrane protein20.60
    JS4709FTL_1328FTT0583fopAOuter membrane-associated protein>50.49
    JS1083FTL_1354FTT0759Membrane protein40.46
    JS4622FTL_1414FTT0807capATransmembrane HSP60 family protein21.10
    JS2531FTL_1415FTT0806capCCapsular polyglutamate biosynthesis protein CapC20.93
    JS2512FTL_1416FTT0805capBCapsular polyglutamate biosynthesis protein CapB31.21
    JS1091FTL_1475FTT1314cType IV pilus fiber building block protein20.57
    JS4638FTL_1678FTT0101Membrane protein10.61
Intracellular survival and stress response
    JS1967FTL_0094FTT1769CclpBEndopeptidase Clp ATP-binding chain B>50.46
    JS2797FTL_0111FTT1359ciglAIntracellular growth30.77
    JS4621FTL_0112FTT1358ciglBIntracellular growth20.96
    JS3579FTL_0113FTT1357ciglCIntracellular growth51.00
    JS3200FTL_0519FTT1606minDDivision inhibitor ATPase10.79
    JS2866FTL_0520FTT1605minCSeptum site-determining protein20.69
    JS1816FTL_0891FTT0623tigMolecular chaperone>50.43
    JS2466FTL_0892FTT0624clpPATP-dependent Clp protease subunit P10.90
    JS2488FTL_0893FTT0625clpXATP-dependent Clp protease subunit X20.72
    JS1838FTL_0894FTT0626lonATP-dependent protease Lon>50.68
    JS1216FTL_1097FTT1102Macrophage infectivity potentiator20.67
    JS4549FTL_1392FTT1471cdeaDCold shock DEAD box protein A40.64
    JS4653FTL_1473FTT1312cuvrADNA excision repair enzyme, subunit A10.75
    JS2712FTL_1504FTT0721ckatGCatalase20.70
Metabolism and biosynthesis
    JS1849FTL_0010FTT1748glpEThiosulfate sulfurtransferase10.95
    JS2839FTL_0193FTT0283cyoCCytochrome O-ubiquinol oxidase subunit III11.05
    JS3158FTL_0382FTT0881crocEAmino acid permease20.39
    JS4576FTL_0387FTT0884caspC1Aspartate aminotransferase11.07
    JS2511FTL_0483FTT0413cglgB1,4-α-Glucan branching enzyme10.93
    JS2367FTL_0485FTT0415glgCGlucose-1-phosphate adenylyltransferase10.81
    JS3699FTL_0525FTT1600cfumAFumerate hydratase20.56
    JS2301FTL_0584FTT1530fadBAcyl-CoA-binding protein41.46
    JS4239FTL_0789FTT1165caspC2Aspartate aminotransferase>50.71
    JS4275FTL_0846FTT1117cIsochorismatase hydrolase family protein10.62
    JS4467FTL_0960FTT0684CsthASoluble pyridine nucleotide transhydrogenase>50.89
    JS1239FTL_1240FTT0963caroGPhospho-2-dehydro-3-deoxyheptonate aldolase>50.34
    JS2226FTL_1266FTT0941clipPLipase/esterase10.89
    JS2758FTL_1273FTT0936cbioF8-Amino-7-oxononanoate synthase10.77
    JS2559FTL_1274FTT0935cbioCBiotin synthesis10.95
    JS4610FTL_1275FTT0934cbioDDethiobiotin synthetase50.90
    JS2717FTL_1419FTT0802cphBCyanophycinase10.90
    JS2632FTL_1553FTT0504csucCSuccinyl-CoA synthetase beta chain10.75
    JS2228FTL_1554FTT0503csucDSuccinyl-CoA synthetase alpha chain10.60
    JS2292FTL_1701FTT1631cglpXFructose-1,6-bisphosphatase>50.57
Transcription, translation, and cell separation
    JS3170FTL_R0003FTTr0716S rRNA16S rRNA>50.80
    JS3560FTL_R0004FTTt19tRNA-IletRNA-isoleucine10.73
    JS3583FTL_0257FTT0346rpmJ50S ribosomal protein L3610.41
    JS4647FTL_0428FTT0908parBChromosome partition protein B10.74
    JS3754FTL_0456FTT0390crpsU130S ribosomal protein S2110.79
    JS2299FTL_0616FTT1442crpoA2DNA-directed RNA polymerase, α subunit10.50
    JS4292FTL_0768FTT1179bipAGTP-binding translational elongation factor Tu and G family protein10.84
    JS3069FTL_0899FTT0631hflXProtease, GTP-binding subunit10.77
    JS3240FTL_0903FTT0633hflKProtease modulator10.59
    JS3165FTL_0928FTT0654elbBDJ-1/PfpI family protein30.90
    JS3181FTL_0950FTT0675rplY50S ribosomal protein L2530.90
    JS4305FTL_1030FTT1056crluBRibosomal large subunit pseudouridine synthase B10.59
    JS4451FTL_1393FTT1472cppiCPeptidyl-prolyl cis-trans isomerase or parvulin11.05
    JS3773FTL_1404FTT0820rplT50S ribosomal protein L2010.47
    JS3636FTL_1452FTT0773rpmA50S ribosomal protein L2710.67
    JS2817FTL_1461FTT0766deoDPurine nucleoside phosphorylase10.20
    JS2840FTL_1474FTT1314cgreATranscription elongation factor>50.43
    JS1379FTL_1601FTT0463yibKtRNA/rRNA methyltransferase11.00
    JS3633FTL_1912FTT0183crpsA30S ribosomal protein S110.46
Substrate binding/transport
    JS4252FTL_0133FTT0249feoBFerrous iron transport protein11.0
    JS2678FTL_0617FTT1441bfrBacterioferritin20.95
    JS3451FTL_0837FTT1125metQd-Methionine-binding transport protein10.68
    JS3166FTL_1233FTT0968cAmino acid antiporter10.69
    JS4295FTL_1458FTT0769secAPreprotein translocase, subunit A10.83
    JS1673FTL_1528FTT0708Major facilitator superfamily transport protein20.34
    JS4666FTL_1622FTT0444Multidrug transporter11.06
    JS4221FTL_1672FTT0105cTransporter AcrB/AcrD/AcrF family10.71
    JS1500FTL_1750FTT0138secEPreprotein translocase, subunit E10.79
    JS3225FTL_1936FTT0209cPeriplasmic solute-binding family protein10.90
    JS3948FTL_1947FTT1782cyjjkABC transporter ATP-binding protein20.50
Others
    JS4680FTL_0012FTT1750recARecombinase A protein10.83
    JS3972FTL_0337FTT0843Unknown11.00
    JS1488FTL_0430FTT0910Unknown40.66
    JS1048FTL_0439FTT0918Unknown>51.24
    JS3952FTL_0440FTT0920Transposase10.95
    JS3638FTL_0514FTT1611Unknown10.84
    JS4714FTL_0663FTT1400cUnknown10.70
    JS4635FTL_0723FTT1221Unknown10.57
    JS3434FTL_0803FTT1152Unknown20.78
    JS1020FTL_1075FTT1015Unknown10.71
    JS4619FTL_1225FTT0975Unknown10.94
    JS3599FTL_1623FTT0443Unknown10.74
    JS2308FTL_1832FTT0029cUnknown10.61
    JS4698FTL_1867FTT1726yegQProtease11.06
  • a A single mutant for each ORF is shown when multiple independent mutants were identified for the ORF.

  • b The ORF designations are adopted from the annotations of the LVS genome (accession no. AM233362). For the genes with multiple alleles (the iglABC and 16S rRNA genes), the precise alleles with transposon interruption were not determined. Only one of the possible insertion sites is listed.

  • c The ORF designations are adopted from the annotations of the Schu S4 genome (accession no. NC_006570). NA indicates the absence of the LVS ORF ortholog in the Schu4 genome.

  • d The gene designations and functions are adopted from the annotated genome sequences of LVS (accession no. AM233362) and Schu S4 (accession no. NC_006570). CoA, coenzyme A.

  • e Independent mutants with insertions at different positions of each ORF.

  • f The in vitro GI was calculated based on changes in the OD600 of each culture within the first 18 h of cultivation as described in Materials and Methods.