TABLE 2.

Antibodies elicited by either nCD or rCD recognize homologous and heterologous isolates of M. catarrhalisa

M. catarrhalis isolateWhole-cell titer of wk 6 antiserum elicited by CD (μg)b
nCD (1)rCD (5)
O35E9,64430,012
TTA245,09967,955
430:3457,25842,596
216:969,61469,881
111:21013,32264,955
1230:35911,47391,943
  • a Mice were immunized with either nCD or rCD with MPL plus alum as adjuvant.

  • b Whole-cell titers were the reciprocal of the serum dilution with an absorbance at 405 nm of 0.1 extrapolated from a linear plot of the logarithm of absorbance versus the logarithm of serum dilution. A whole-cell titer of <50 was obtained for the preimmune serum and a negative-control antiserum from mice immunized with 5 μg of an induced E. coli BL21(DE3)/pRSET/Kanr cell lysate.