Table 1.

Strains and plasmids used

Strain or plasmidRelevant genotypeReference or source
L. pneumophilaa
 Lp01Philadelphia-1 rpsL hsdR 2
 Lp02Lp01thyA 2
 HL019Lp01/pMS8This work
 HL900Lp01 dotG?This work
 HL1000Lp01 dotO2 This work
 HL1005Lp01dotO2/pMS8This work
 HL1009Lp01dotO2/p1415This work
 HL1011Lp01dotO2/p1AThis work
 HL1012Lp01dotO2/p2AThis work
 HL1300Lp01 Dot This work
 HL1400Lp01 dotO1 This work
 HL1405HL1400/pMS8This work
 HL1419HL1400/p1415This work
 HL1421Lp01dotO1/p1AThis work
 HL1422Lp01dotO1/p2AThis work
 HL1600Lp01 Dot This work
 HL1700Lp01dotH1This work
 HL1705HL1700/pMS8This work
 HL1719HL1700/p1713This work
 HL1725HL1700/pH3This work
 HL056Lp01dotIΔ1 This work
 HL057HL056/pMS8This work
 HL059HL056/pI1This work
E. coli
 DH5α supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 49
 DH5α (λpir)DH5α (λpir)tet::Mu recA 24
 XL1-BlueF′::Tn10 proA+B+ lacI qΔ(lacZ)M15/recA1 endA1 gyrA96(Nalr) thi hsdR17 (rK mK +) supE44 relA1 lac 5
 MT607 recA56 pro-82 thi-1 hsdR17 supE44, RK600 14
Plasmids
 pMS4 oriRSF1010 RP4 mob kan 39
 pMS8pMS4sacB 37
 p1415pMS8proP+ dotO+ This work
 p1ApMS8 proP′ This work
 p2ApMS8dotO+ This work
 p1713pMS8dotH+ dotI+ This work
 pH3pMS8 dotH+ This work
 pI1pMS8 dotI+ This work
 pSR47 oriTRP4 oriR6Kkan 27
 pSR47SpSR47sacB 45a
 pHJKpSR47sdotH+ dotJ+ dotK+ This work
  • a All were derived from Philadelphia-1, serogroup 1 strain Lp01 (CDC, Atlanta, Ga.).