Table 3.

Survival of strain 15029 in nonopsonic phagocytosis assays under various growth conditionsa

Condition of growthMAb 1B2 binding (OD405)b% Survivalc
Without CMP-NANA0.8115
CMP-NANA0.5529
CMP-NANA and neuraminidase0.8415
  • a Decreased binding of MAb 1B2 indicates that more sialic acid bound to LNnT.

  • b As measured by ELISA.

  • c The phagocytosis assays were serum free. The reaction tubes contained bacteria, neutrophils, and GB. Control tubes contained bacteria, neutrophils, and GB that were incubated at 4°C. Percent survival in control tubes was 98 to 104%.