TABLE 3.

Cytokine release in VDC subpopulationsa

VDC subpopulationCytokine expression (pg/ml)b
IL-10TNF-αIL-12IL-2
CD4+ CD86+ CD80+ CD134L+400 ± 28240 ± 21250 ± 23250 ± 22
CD4+ CD86+ CD80 CD134L+250 ± 20150 ± 19150 ± 20240 ± 26
CD4 CD86 CD80 CD134L+360 ± 24600 ± 30*550 ± 27*250 ± 25
  • a A total of 2 × 105 highly purified OX62+, CD4+ CD5+ CD86+ CD80+ CD134L+, CD4+ CD5+ CD86+ CD80 CD134L, and CD4 CD5 CD86 CD80 CD134L+ VDCs from noninfected and C. albicans-infected rats were cultured in complete medium in 96-well plates at 37°C in 5% CO2. After 36 h, the supernatants were collected. The IL-2, TNF-α, IL-2, and IL-10 release was measured by ELISA.

  • b Results are the mean ± standard deviation of two separate experiments. *, P ≤ 0.01 by Student's t test.