TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
B. abortus strains
    2308Wild typeB. Deyoe
    Strain 19Vaccine strainNVSL
    BAΔasp24::kanΔasp24::KmThis work
    BAΔasp24Δasp24This work
    BAΔvirB2::kanΔvirB2::Km (polar)This work
    BAΔvirB2ΔvirB2 (nonpolar)This work
    BAΔmanBAΔmanBAThis work
B. melitensis strains
    16MWild typeFicht lab
    Rev 1Vaccine strainM. Banai
    BMΔasp24::kanΔasp24::KmThis work
    BMΔasp24Δasp24This work
    BMΔvirB2::kanΔvirB2::Km (polar)This work
    BMΔvirB2ΔvirB2 (nonpolar)This work
    BMΔmanBA::kanΔmanBA::KmThis work
    BMΔmanBAΔmanBAThis work
E. coli strains
    DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk mk+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
    Top10F mcrA Δ(mrr-hsdRMS-mcrBC) φ 80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
    DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) φ 80lacZΔM15 ΔlacX74 recA1 endA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) nupGInvitrogen
Plasmids
    pBluescript KSColE1, blaStratagene
    pKD4FLP/FRT, KmrB. Wanner
    pEX18ApsacB blaH. Schweizer
    pMMKBTAF101/TAF104 cloned into pEX18ApThis work
    pMMK8TAF101/TAF104 cloned into pBluescriptThis work
    pMMK16pMMK8 separated by TAF300/TAF301 (kanamycin resistance)This work
    pMMK29TAF356/TAF359 cloned into pBluescriptThis work
    pMMK31TAF356/TAF359 cloned into pEX18ApThis work
    pMMK33pMMK29 separated by TAF204/205 kanamycin resistance geneThis work
    pAV2.2Plasmid to make marked virB2 deletion13
    pAS1.1Plasmid to make unmarked virB2 deletion13