TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or source
E. coli strains
    DH5αHost strain for cloning experiments34
    HB101Host strain essential for propagating plasmids carrying mutated Francisella genes used for electroporation of Francisella34
Francisella tularensis strains
    LVS no. 11F. tularensis LVS10
    LVS figA::np-kanMutant of LVS with promoterless kan cartridge inserted in figA geneThis study
    LVS figA::np-kan(pKD107)LVS figA::np-kan containing pKD107 with F. novicida U112 figA gene; expresses FigA proteinThis study
Francisella novicida strains
    U112Wild-type strain of F. novicida26
    U112 figA::np-kanMutant of U112 with promoterless kan cartridge inserted in figA geneThis study
    U112 figA::np-kan(pKD107)U112 figA::np-kan containing pKD107 with F. novicida U112 figA gene; expresses FigA proteinThis study
    U112 figA::np-kan(pKD108)U112 figA::np-kan containing pKD108 with F. tularensis LVS figA gene; expresses FigA proteinThis study
    U112 figA::np-kan(pFNLTP-CAT)U112 figA::np-kan containing vector pFNLTP-CATThis study
Plasmids
    pACYC184Broad-host-range cloning vector; Chlorr TetrNew England Biolabs
    pKD101pACYC184 with 2.2-kb fragment containing figA flanking regionsThis study
    pKD101-np-kanpKD101 with promoterless kan cartridge inserted into KpnI siteThis study
    pFNLTP6Shuttle vector for Francisella; Kanr Ampr24
    pKD105pFNLTP6 with F. tularensis LVS figA gene inserted into BamHI siteThis study
    pKD108pKD105 with ΔEcat cartridge inserted into XhoI site; this vector backbone containing the ΔEcat cartridge is the same as that of pFNLTP-CATThis study
    pFNLTP-CATpFNLTP6 with ΔEcat cartridge inserted in XhoI site; Kanr Ampr ChlorrThis study
    pKD107pFNLTP-CAT carrying F. novicida U112 figA geneThis study