TABLE 1

Microbial strains and plasmids used in this study

Strain or plasmidUnique IDRelevant genotypeReference or source
Strains
    C. albicans
        SC5314UB1843Wild typeNeil Gow, University of Aberdeen
UB1941Δals339
UB1940Δals3 pUL.als339
    S. cerevisiae
        BY4742UB2156pBC542-ALS3sm40
    S. agalactiae
        NEM316UB1931Wild typeShaynoor Dramsi, Institut Pasteur
        515UB2410Wild typeVictor Nizet, University of California, San Diego
        515UB2873ΔbspCThis study
    L. lactis
        NZ9800UB2635pMSP12
UB2658pMSP.bspA12
UB2659pMSP.bspCThis study
Plasmids
    pMSP7517E. coli-Enterococcus shuttle vector containing Enterococcus faecalis prgB under the control of the nisA promoter; erythromycin resistance54
    pMSP.bspCpMSP7517-derived plasmid containing bspC from GBS 515 in place of prgB
    pR326Chloramphenicol resistance58
    pHY304Temp-sensitive E. coli-Streptococcus shuttle vector53