TABLE 1.

Enterococcal strains and plasmids used in this study

Strain or plasmidCharacterizationReference or source
Strains
    E. faecalis V583Reference strain, fully sequenced32
    E. faecalis ATCC 29212Reference strain
    E. faecalis 12030Clinical isolate, strong biofilm producer18
    E. faecalis 12030ΔdltAdltA mutantThis study
    E. faecalis 12030 R-wtWild-type revertant strainThis study
    E. faecalis 12030ΔdltA/pEUdltAStrain complemented with the dltA geneThis study
    E. faecalis 12030ΔdltA/pEUdltABStrain complemented with the dltA and dltB genesThis study
    E. coli DH5αGram-negative cloning host
    E. coli TOP10Gram-negative cloning hostInvitrogen
Plasmids
    pCASPERGram-positive, temp-sensitive mutagenesis vector4
    pCRII-TOPOGram-negative cloning vectorInvitrogen
    pEU327Gram-positive expression vector11
    pCASPER/ΔdltAThis study
    pEUdltAExpression vector carrying the dltA geneThis study
    pEUdltABExpression vector carrying the dltA and dltB genesThis study