TABLE 2.

Primers used in this study

Primer nameSequence (restriction enzyme engineered)Fragment
TAF1015′-GGAATTCGGCAAAGCGAGTGGGTGATTAG-3′(EcoRI)asp24 upstream
TAF1025′-CGGGATCCTGAGCAAGTGCGGGAATAGC-3′(BamHI)asp24 upstream
TAF1035′-CGGGATCCTGGGAATGGAGCGGCTTTAG-3′(BamHI)asp24 downstream
TAF1045′-GCTCTAGATTTGAACACTTGGCGATAGCG-3′(XbaI)asp24 downstream
TAF3005′-CGGGATCCCGCACGTCTTGAGCGATTGTGTAGG-3′(BamHI)Kan cassette
TAF3015′-CGGGATCCCGGGACAACAAGCCAGGGATGTAAC-3′(BamHI)Kan cassette
TAF3565′-CGGGATCCCTGGAGGAAAACAATCTGGG-3′(BamHI)manBA upstream
TAF3575′-AAGACGGCGCGCCCGAACCTGTATCTGCCTG-3′(AscI)manBA upstream
TAF3585′-GTTCGGGCGCGCCGTCTTAACCCAAAACCGCTTCGTA-3′(AscI)manBA downstream
TAF3595′-GCTCTAGAGGGTTTTCTGATCGATCTGGTAGC-3′(XbaI)manBA downstream
TAF2045′-GGCGCGCCACGTCTTGAGCGATTGTGTAGG-3′(AscI)Kan cassette
TAF2055′-GGCGCGCCGGACAACAAGCCAGGGATGTAAC-3′(AscI)Kan cassette