TABLE 1.

Phenotype of VDC subsetsa

MAb subsetMFI (mean ± SD)b
OX62+ CD4+OX62+ CD4
NoninfectedInfectedNoninfectedInfected
CD599.1 ± 4.3126.1 ± 3.2*
TCR-α/β
TCR-γ/δ
CD8α
CD11b16.4 ± 1.263.5 ± 2.9*9.7 ± 0.9106.2 ± 3.9*
Class II RT1B6.6 ± 0.927.7 ± 1.2*24.9 ± 1.372.9 ± 2.7*
CD8049.9 ± 2.0*
CD8617.3 ± 0.9555.5 ± 8.9*
OX40L2.0 ± 0.262.8 ± 1.3*537.4 ± 10.0*
  • a Freshly isolated OX62+ VDCs from noninfected and C. albicans-infected rats were double stained with FITC- or PE-conjugated rat anti-CD4 and FITC- or PE-conjugated MAbs against the molecules listed in the left column.

  • b Data are expressed as the MFI on gated CD4+ and CD4 VDC subsets, corrected by subtracting the MFI of the isotype control Abs and are the mean ± standard deviation (SD) of three separate experiments. Statistical analysis was performed by comparing the MFIs of OX62+ CD4+ and OX62+ CD4 VDCs from noninfected versus infected rats. *, P < 0.01 by Student's t test.