TABLE 1.

Selected genes stimulated or repressed by tobramycin in CFBE/PA14 biofilmsa

GeneFold changeDescription
PQS
    PA0996−2.5pqsA (biosynthesis of PQS)
    PA0997−19.5pqsB (biosynthesis of PQS)
    PA0998−15.0pqsC (biosynthesis of PQS)
    PA0999−10.1pqsD (biosynthesis of PQS)
    PA1000−13.8pqsE (biosynthesis of PQS)
Alginate
    PA0762+8.6algU (sigma factor)
    PA0763+6.5mucA (anti-sigma factor)
    PA5262+3.6algZ (alginate biosynthesis protein)
    PA5255+2.8algQ (alginate biosynthesis regulator)
    PA5261+2.8algR (alginate biosynthesis regulator)
    PA5253+2.1algP (alginate biosynthesis regulator)
    PA0764+2.0mucB (alginate biosynthesis negative regulator)
Iron
    PA3531+16.5bfrB (bacterioferritin)
    PA4764+4.2fur (ferric uptake regulation)
    PA0362+2.1fdx1 (ferridoxin)
    PA4131−2.0Probable iron-sulfur protein
    PA3491−2.0Probable ferredoxin
    PA5217−2.3Probable binding protein component of ABC iron transporter
    PA5216−2.4Probable permease of ABC iron transporter
    PA3812−2.5iscA (probable iron-binding protein)
    PA3813−2.8iscU (probable iron-binding protein)
    PA2399−3.1pvdD (pyoverdine synthetase)
    PA3809−3.4fdx2 (ferridoxin)
    PA2386−4.2pvdA (pyoverdine synthesis)
    PA2426−4.3pvdS (sigma factor, iron responsive)
    PA4231−4.4pchA (salicylate biosynthesis, pyochelin biosynthesis)
    PA2397−4.8pvdE (pyoverdine synthesis)
    PA4225−6.1pchF (pyochelin synthetase)
    PA4226−7.7pchE (pyochelin synthesis)
    PA4228−13.9pchD (pyochelin synthesis)
    PA4221−25.3fptA (pyochelin receptor precursor)
    PA2398−26.3fpvA (ferripyoverdine receptor)
    PA4229−28.9pchC (pyochelin synthesis)
    PA4230−29.9pchB (salicylate biosynthesis, pyochelin synthesis)
Magnesium
    PA4635+8.2hypothetical MgtC protein
    PA0913+3.9mgtE (magnesium transport)
    PA4825+2.8mgtA (magnesium transport)
  • a The table shows the overall trends of several metabolic activities. Full microarray data can be found in the supplemental information.