TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
A. salmonicida subsp. salmonicida strains
    A449Virulent, Cmr 29
    02-02A449 flpA::Km, Cmr KmrThis study
    02-06Lab-derived mutant of A449, O side chain negative, surface layer secretor, Cmr 43
    02-10A449 tapA::KO, Cmr AprThis study
    03-01A449 flpA::Km tapA::KO, Cmr Kmr AprThis study
P. aeruginosa PAKWild typeD. Bradley
E. coli strains
    TOP10K-12Invitrogen
    DH10BK-12 for large plasmidsInvitrogen
    EC100D pir-116K-12 pir-116Epicenter
    BW20767K-12 pir+, conjugation positive 28
Plasmids
    pUTkm1Cloning vector, Apr Kmr 13
    pCRScript-AmporiE1, AprStratagene
    pAH34oriR6Kγ mobRP4, Apr 28
    pKOpAH34 with tmRNA tagThis study
    pKO-tapAInternal fragment of tapA in pKO, in frame with tmRNA tagThis study
    pGP704oriR6Kγ mobRP4, Apr 21
    pJW109-pirpGP704 with flpA-flanking regions surrounding kanamycin resistance cassette, Kmr AprThis study
    pMMB67EHLow-copy-number, broad-host-range expression vector, mobRSF1010, Apr 20
    pMMB67EH.KmpMMB67EH with Apr replaced by KmrThis study
    ptapA.KpMMB67EH.Km carrying complete tapA geneThis study
    pflp1.KpMMB67EH.Km carrying complete flp-1 geneThis study
    pBACe3.6Large insert vector, Cmr 19
    pAs01a04Derivative of pBACe3.6 with 71.2-kb insert carrying the flp locusThis study