TABLE 1.

Strains used in this study

StrainDescriptionReference or source
V. cholerae strains
    AC51El Tor C6709, Smr39
    AC66C6709 lacZ::res-tet-res7
    AC1901C6709 pMMB67EH::vieA-His6This work
    AC1902C6709 pMMB67EH::VCA0956(pVdcA)This work
    AC1903C6709 lacZ::res-tet-res pMMB67EHThis work
    AC2131C6709 cdpA::pGP704This work
    AC2280C6709 ΔvpsRThis work
    AC2284C6709 ΔvpsR pMMB67EH::vieA-His6This work
    AC2286C6709 ΔvpsR pVdcAThis work
    AC2365C6709 lacZ::res-tet-res pMMBneoThis work
    AC2377C6709 cdpA::pGP704 pMMBneo::cdpA-His6This work
    AC2380C6709 pMMBneo::cdpA-His6This work
    AC2389C6709 pMMB67EH::VCA0956(E258A) pVdcA(I)This work
    AC2390C6709 pMMB67EH::vieA(E170A)This work
    AC2395C6709 lacZ pVdcAThis work
    AC2434C6709 cdpA::pGP704 pMMBneoThis work
    AC2435C6709 ΔvpsR pMMBneoThis work
    AC2483C6709 toxT::tnpR lacZ::res-tet-res pVdcAThis work
    AC2484C6709 toxT::tnpR lacZ::res-tet-res pVdcA(I)This work
    AC2595C6709 lacZ::res-neo-sacB-resThis work
    AC2606C6709 cdpA::tnpR lacZ::res-sacB-neo-resThis work
    AC2655C6709 pMMBneo::cdpA(AVGAW)-His6This work
    AC2658C6709 cdpA::pGP704 pMMBneo::cdpAΔEAL-His6This work
    AC2659C6709 cdpA::pGP704 pMMBneo::cdpA(AVGAW)-His6This work
E. coli strains
    DH5αF Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1Laboratory strain
    DH5αλPirF Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ::pirLaboratory strain
    SM10λPirthi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pirLaboratory strain
    AC575DH5α pMMB67EH33
    AC751DH5αλPir pCVD442lac11
    AC2364DH5α pMMBneo35
    AC1817DH5α pMMB67EH::vieA-His648
    AC1835DH5α pMMB67EH::vieA(E170A)-His648
    AC1887DH5α pVdcAThis work
    AC2129SM10λPir pGP704::′cdpAaThis work
    AC2142SM10λPir pCVD442lac::ΔvpsRThis work
    AC2376DH5α pMMBneo::cdpA-His6This work
    AC2386DH5α pVdcAThis work
    AC2528SM10λPir pRes35, 44
    AC2599SM10λPir pIVET5ncdpAThis work; 44
    AC2641DH5α pMMBneo::cdpAΔEAL-His6This work
    AC2642DH5α pMMBneo::cdpAAVGAW-His6This work
  • a An internal fragment of cdpA was cloned in order to make the plasmid insertion.