Table 1.

Kinetics of apoptosis in BAL fluid and lung tissue ofB. pertussis-infected mice

Days after infectionMean ± SEM (n = 3–12)
BAL fluidLung tissue, apoptotic cellsc
Apoptotic nuclei (%)aPhagocytosis (%)b
18323H1832318HS1918323H1832318HS1918323H1832318HS19
24.3 ± 0.97.8 ± 0.53.3 ± 0.30.3 ± 0.10.4 ± 0.10.1 ± 0.129.3 ± 5.886.3 ± 39.4NDd
71.8 ± 0.113.3 ± 2.1e6.3 ± 0.7e,f0.3 ± 0.11.3 ± 0.3e1.2 ± 0.4e79.2 ± 11.1290.7 ± 84.4e74.7 ± 18.5f
142.5 ± 0.617.1 ± 3.8e1.4 ± 0.1e0.4 ± 0.15.3 ± 1.2e0.3 ± 0.1f44.0 ± 21.4343.7 ± 108.7e23.3 ± 5.6f
212.4 ± 0.410.7 ± 1.7eND0.3 ± 0.16.0 ± 1.8eND38.3 ± 19.952.7 ± 24.9ND 
  • a Detected by flow cytometry.

  • b Proportion of alveolar macrophages ingesting apoptotic cells or their bodies (light microscopy).

  • c Number of apoptotic cells in 20 fields of the alveolar compartments (TUNEL technique).

  • d ND, not determined.

  • e P < 0.05 compared to 18323H-infected mice.

  • f P < 0.05 compared to 18323-infected mice.