Table 1.

IL-8 accumulation by GEC in response to periodontal bacteriaa

Bacterium IL-8 level (ng/ml)b
Porphyromonas gingivalis 0 ± 0
Fusobacterium nucleatum 383 ± 25
Fusobacterium nucleatum + MY4c 0 ± 0
Fusobacterium nucleatum + P. gingivalis 0 ± 0
Neisseria flavescens 353 ± 28
Neisseria flavescens + P. gingivalis 0 ± 0
Haemophilus parainfluenzae 383 ± 11
Haemophilus parainfluenzae + P. gingivalis 0 ± 0
Eikenella corrodens 328 ± 37
Eikenella corrodens + P. gingivalis 0 ± 0
Leptrotrichia buccalis 400 ± 14
Leptrotrichia buccalis + P. gingivalis 0 ± 0
  • a IL-8 in the culture supernatant was assayed after 18 h of incubation with bacteria (108cells/well). In experiments which contained P. gingivalisand other bacteria, the other bacteria (108) and P. gingivalis bacteria (106) were mixed prior to addition to the GEC.

  • b The means and standard deviations from three separate experiments are reported.

  • c MY4 is an anti-CD14 monoclonal antibody and was added at 40 ng/ml.