Table 2.

Partial listing of top matches to known proteins in GenBank identified by BLASTx homology searches

ContigESTGene nameBLASTx PvalueaBLASTx high scoreaNo. of bradyzoite ESTsNo. of tachyzoite ESTs
Genes for metabolic enzymes
bs4_98TgESTzz32g04 Deoxyribose phosphate aldolase5.7 × 10−7 115220
bs4_320TgESTzz34g03 Apurinic endonuclease1.2 × 10−42 124140
bs4_10TgESTzz54b03 Ketoacyl reductase2.1 × 10−6 81120
bs4_48TgESTzz33a12 Enolase4.1 × 10−81 702130
bs4_619TgESTzz66d07 NTPase homolog6.1 × 10−22 21110
bs4_26/507TgESTzz38f05 Oxoglutarate-malate translocator4.3 × 10−16 116100
bs4_760TgESTzz59c08 Dihydroorotate dehydrogenase9.8 × 10−45 20410
bs4_826TgESTzz35c04 Pyrroline-5-carboxylate reductase1.4 × 10−20 17810
bs4_359TgESTzz26e05 Methionine aminopeptidase2.1 × 10−65 39440
bs4_246TgESTzz36a03 Molybdenum cofactor4.9 × 10−16 17640
 Biosynthetic precursor E
bs4_435TgESTzz64b03 Hsc-70-interacting protein of SCN63.5 × 10−8 12570
bs4_398TgESTzz68f03 Heat shock protein1.5 × 10−38 29030
bs4_57TgESTzz35f09 Thiolesterase3.0 × 10−18 13530
bs4_286TgESTzz37c01 Thioredoxin9.6 × 10−19 15430
bs4_201TgESTzz54c12 Calcium-independent phospholipase A25.6 × 10−37 18531
bs4_547TgESTzz68h05 Glutathione peroxidase5.0 × 10−13 11830
bs4_197TgESTzz35a02 Enoyl coenzyme A hydratase6.4 × 10−13 11120
bs4_885TgESTzz34c10 Cyclic AMP protein kinase regulatory chain1.2 × 10−9 11210
bs4_182TgESTzz46e12 SNF2L transcription activator1.7 × 10−30 12770
bs4_09TgESTzz34g08 Protein phosphatase 2C6.3 × 10−9 9350
bs4_624TgESTzz33b10 Karyopherin alpha-2 subunit7.8 × 10−74 25320
bs4_802TgESTzz36d09 Cyclophilin1.2 × 10−47 34910
bs4_713TgESTzz68f05 Cul-35.0 × 10−16 14310
bs4_791TgESTzz38e01 Golgi adapter Ap-1 (47 kDa)5.7 × 10−52 26010
bs4_445TgESTzz68f10 Coatamer beta′ subunit1.1 × 10−14 11320
Parasite-specific genes
bs4_31TgESTzz67e09SAG4homolog2.6 × 10−33 126210
bs4_309TgESTzz69c05ROP8homolog0.00859092
bs4_537TgESTzz48e02ROP8homolog1.1 × 10−16 17920
bs4_305TgESTzz34c07ROP2homolog3.1 × 10−12 9761
bs4_68TgESTzz35e11P22 (SAG2) homolog0.71  71120
bs4_88TgESTzz53c11 Sporozoite antigen 19 kDa3.4 × 10−47 222248
  • a The BLASTx P value indicates the probability that the match could have occurred by chance. The BLASTx high score is a measure of the best, ungapped alignment (>70 is often significant). Neither the P value nor the high score alone can be relied upon to always pick up significant matches, and so both are presented here.