Table 1.

Bacterial strains and plasmids used in this study

Strain, phage, or plasmidRelevant propertiesSource or reference
P. aeruginosa strains
 PA103Wild-type cytotoxic respiratory clinical isolate 1, 27
 Noncytotoxic mutants of PA103Summarized in Table2 22
E. coli strains
 S17-1 thi pro hsdR recA RP4-2(Tet::Mu) (Km::Tn7) 47
 XL1-Blue MRF′Δ(mcrA)183 Δ(mcrCB hsdSMR mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacI q ZΔM15Tn10(Tetr)]Stratagene Corp.
Plasmids and cosmids
 pBR322Cloning vectorGibco BRL
 pBluescriptIICloning vectorStratagene Corp.
 pLAFR5SK1Cosmid vector; pLAFR5 (23) with improved polylinker siteL. Shapiro
 4-7-HpLAFR5SK1 cosmid with PA103 genomic DNA insertThis study
 4-11-HpLAFR5SK1 cosmid with PA103 genomic DNA insertThis study
 5-9-EpLAFR5SK1 cosmid with PA103 genomic DNA insertThis study
 pAH830pBluescriptII containing the 2.0-kbSmaI-SmaI fragment of cosmid 4-7-HThis study
 pAH831pBluescriptII containing the 8.0-kbEcoRI-EcoRI fragment of cosmid 4-7-HThis study