Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
L. pneumophila serogroup 1 (Philadelphia)ATCC 33152; type strainCDCa
L. longbeachaeserogroup 1ATCC 33462; type strainCDC
L. longbeachae serogroup 2ATCC 33484; type strainCDC
 A5H5 (L. longbeachae serogroup 1)Australian clinical isolateThis study
 B10ATCC 33462 L. longbeachae serogroup 1 strain with a mip deletion mutationThis study
 B8 L. longbeachae serogroup 1 A5H5 with a mip deletion mutationThis study
 B8.22Strain B8 complemented with plasmid SKW27This study
E. coli DH5αFφ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rk mk +) deoR thi-1 supE44 λgyrA96 relA1 20
E. coliS17-1 recA derivative of E. coli294 (hsdR Pro) with RP4-2Tc::Mu (Ap Km Nm Tc::Mu) Km::Tn7 in the chromosome 39
Plasmids
 pGEM-7Zf(−)Apr cloning vectorPromega
 pUC18KpUC18 with the aphA-3 Kmrresistance cassetteP. Sansonetti, reference 31
 pCACTUSCmr cloning vector containingsacB and a temperature-sensitive repliconC. A. Clark
 pIMVS26pGEM with ca. 8-kb fragment of L. longbeachae serogroup 1 ATCC 33462 genomic DNAThis study
 pIMVS27pGEM carrying a SacI fragment of pIMVS26This study
 pIMVS28pGEM containing deletedmip gene generated in pIMVS27This study
 pCACTUS49pCACTUS containing deleted mip gene fragment from pIMVS28This study
 pCACTUS50Derivative of pCACTUS49 containing Kmr from pUC18K and mobdeletionThis study
 pWKS130Kmr cloning, sequencing vector 46
 pIMVS29pWKS130 containing entire mip gene on SacI fragment from pIMVS27This study
  • a CDC, Centers for Disease Control and Prevention, Atlanta, Ga.