TABLE 3.

Repression of S. flexneri sit and mntH expression by manganese and iron

Added metalaRFU/OD650b of SM100 carrying plasmid:
pEG2 (sit-gfp)pRJ8 (mntH-gfp)
None2,614731
FeSO4241158
MnSO4132124
FeSO4 and MnSO48289
  • a Metal concentrations were 10 μM.

  • b Fluorescence was quantitated by fluorometry (relative fluorescence units [RFU]) and was normalized to cell growth by dividing the fluorescence of the sample by the optical density at 650 nm (OD650). The data presented are the means of three experiments.