TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristic(s)Reference or source
E. coli strains
    DH5αendA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15]43
    SIP879MC4100 mntH::Mud1 aroB33
    SIP943MC4100 mntH::Mud1 aroB mntR33
S. flexneri strains
    SA100S. flexneri wild-type serotype 2a35
    SM100SA100 StrrS. Seliger
    SM166SM100 sitA::cam41
    UR002SM100 mntH::kanThis study
    UR003SM100 sitA::cam mntH::kanThis study
    UR009SM100 oxyR::kanThis study
    UR010SM100 fur::Tn5This study
Plasmids
    pLR29Promoterless GFP plasmid40
    pEG2sitA-gfp fusion on pLR2940
    pRJ8mntH-gfp fusion on pLR29This study
    pLAFR1Cosmid vector10
    pWKS30Low-copy-number cloning vector46
    pSITpLAFR1 carrying SA100 sit operon (pEG1)40
    pMNTHpWKS30 carrying SA100 mntHThis study