TABLE 3.

FACS and RT-PCR analysis of PBMC from three donors cultured for 12 days in the presence of SE/U2 or SE/V

SampleTCR Vβa% of total CD3+ cells in cultureb for donor:
ABC
FACSRT-PCRFACSRT-PCRFACSRT-PCR
Day 0134.524.51.338.81.353.9
1451.11.60.20.710.2
183.14.61.64.91.33.6
213.229.43.341.63.287.6
PHAc133.1210.11.034.11.1311.3
144.681.13.571.22.70.6
180.426.90.59.20.644.6
212.228.61.49.81.848.1
SelU21316.24.77.4611.63.98.9
1454.2856.476.3768.371.3948.8
180.460.90.110.90.030.8
211.2822,130.21.471
SelV131.9671.7690.674.3
143.90.11.230.61.90.3
185.0414.81.8114.81.328.3
215.7630.57.2237.85.440.8
  • a 13, Vβ 13.2 in FACS assay and Vβ 13a in RT-PCR quantification assay; 21, Vβ 21.3 in FACS assay and Vβ 21f in RT-PCR quantification assay.

  • b Values in boldface indicate specific expansions.

  • c Four-day PHA stimulation.