TABLE 1.

Primers used for detection of egc genes and for SElU2 and SElV cloning and sequencing

PrimerLocation within egcaOligonucleotide sequence (5′→3′)b
primer5′1-19GTC CCG TTA GGA GTC ATA C
seo1481-506AGT TTG TGT AAG AAG TCA AGT GTA GA
seo2630-660ATC TTT AAA TTC AGC AGA TAT TCC ATC TAA C
seo3427-449GCA TTG TTT ACA CTA CAT ATT GC
seo4244-270CTG TTT GTT CAA TAG TAA GTA GGA TTG
seo5557-581GTT GAT ACA ATT GAT TTT ACT GTC G
invseo2630-660GTT AGA TGG AAT ATC TGC TGA ATT TAA AGA T
invsem11785-1811GTT CTC CAT TAA CCC AAA GAT TAA TAG
sem11785-1811CTA TTA ATC TTT GGG TTA ATG GAG AAC
sem22085-2110TTC AGT TTC GAC AGT TTT GTT GTC AT
invsei12260-2281CCT ACA CCA ATA TCA CCT TGA G
sei12260-2281CTC AAG GTG ATA TTG GTG TAG G
sei22886-2915GTT ACT ATC TAC ATA TGA TAT TTCGAC ATC
invsei22886-2915GAT GTC GAA ATA TCA TAT GTA GAT AGT AAC
yent13352-3375ACG TAG ATT TGT TTG GGA CAA ACT
yent23502-3529GTG CTG TTA TGT TTT TCT TAT TAG TAG G
invsen13969-3988GAC TCG TCT AAT TGC CAC GT
sen13969-3988ACG TGG CAA TTA GAC GAG TC
sen24415-4444GAT TGA TCT TGA TGA TTA TGA GAA TGA AAG
invsen24415-4444CTT TCA TTC TCA TAA TCA TCA AGA TCA ATC
invseg14979-5003CAT TAC ATT ACC CAT AGT TCC CTT A
seg14979-5003TAA GGG AAC TAT GGG TAA TGT AAT G
seg25514-5541GAA CAA AAG GTA CTA GTT CTT TTT TAG G
invseg25514-5541CCT AAA AAA GAA CTA GTA CCT TTT GTT C
primer3′6163-6189CTT TAA CTT CAT AAA TTA GCA GTA GTC
pQE-FcCCC GAA AAG TGC CAC CTG
PQE-RcGTT CTG AGG TCA TTA CTG G
R-SElU2-1CA GGATCC ATG TTA AAT GGC AAT CCT AAA C CA
R-SElU2-2GC CTGCAG TTA TTT TTT GGT TAA ATG AAC TTC TAC ATT AAT AGA TTT A
R-SElV 1GCA GGATCC GAT GTC GGA GTT TTG AAT CTT AGG
R-SElV 2TAA CTGCAG TTA GTT ACT ATC TAC ATA TGA TAT TTC GAC ATC
  • a Location based on the S. aureus egc sequence (GenBank accession number AFP 285760).

  • b Restriction sites for BamHI and PstI are underlined.

  • c Primer used for sequencing the inserted gene in the pQE-30 vector.