TABLE 1.

Evolution of M. paratuberculosis S-23 numbers in spleen, liver, and lungs as determined by luminometry and classical CFU counting

OrganMouseNo. of bacteriaa
4 wk after infection12 wk after infection
Log10 RLULog10 CFULog10 CFU(h)Log10 RLULog10 CFULog10 CFU(h)
SpleenC57BL/65.29 ± 0.44 (5)5.56 ± 0.58 (5)5.39 ± 0.66 (5)4.59 ± 0.21 (5)5.80 ± 0.11 (5)5.47 ± 0.13 (5)
BALB/c5.16 ± 0.15 (4)5.63 ± 0.19 (4)5.66 ± 0.13 (4)5.23 ± 0.34 (4)6.20 ± 0.52 (3)6.02 ± 0.34 (3)
C.D23.88 ± 0.10 (4)4.70 ± 0.17 (4)4.58 ± 0.19 (4)3.52 ± 0.12 (6)3.97 ± 0.38 (6)4.02 ± 0.28 (6)
LiverC57BL/65.59 ± 0.06 (5)6.68 ± 0.09 (5)6.52 ± 0.10 (5)3.65 ± 0.22 (5)5.48 ± 0.25 (5)5.24 ± 0.25 (5)
BALB/c5.87 ± 0.06 (4)6.62 ± 0.13 (4)6.38 ± 0.16 (4)5.33 ± 0.40 (3)7.07 ± 0.27 (3)6.50 ± 0.18 (3)
C.D24.40 ± 0.14 (4)5.58 ± 0.19 (4)5.42 ± 0.24 (4)3.22 ± 0.10 (6)4.80 ± 0.19 (6)4.60 ± 0.34 (6)
LungsC57BL/63.68 ± 0.17 (5)4.01 ± 0.36 (5)4.07 ± 0.48 (5)3.06 ± 0.07 (5)3.76 ± 0.63 (5)3.34 ± 0.51 (5)
BALB/c3.34 ± 0.02 (4)4.15 ± 0.66 (4)4.06 ± 0.70 (4)3.42 ± 0.71 (3)5.00 ± 0.62 (3)4.59 ± 0.73 (3)
C.D22.90 ± 0.02 (4)4.41 ± 0.71 (4)4.26 ± 0.73 (4)2.95 ± 0.17 (6)2.85 ± 0.40 (6)2.89 ± 0.46 (6)
  • a Organs from individual infected mice were homogenized, and the numbers of bacteria/organ were determined by luminometry or by plating the bacteria on Middlebrook 7H11 agar with [CFU(h)] or without (CFU) hygromycin. The data represent mean (± standard deviation) values for three to six mice (numbers are given in parentheses).