TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or descriptionaSource or reference
Bacterial strains
    H10407ETEC serotype O78:H11, LT+ ST+17
    H10407-SSpontaneous Smr derivative of H10407
    jf1289Isogenic deletion mutant of etpA21
    AAEC191Afim deletion mutant of E. coli K-12 (MG1655)3
    BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Novagen
    jf1668Isogenic deletion mutant of etpA bearing a Cmr cassette in etpAThis study
Plasmids
    pBAD/Myc-His AArabinose-inducible expression plasmid; AmprInvitrogen
    pJL017etpBA cloned into pBAD/myc-His AThis study
    pGPS4Transposon donor; CmrNEBb
    pJMF1019pJL017 containing etpA mutagenized (::Cmr) with the GPS4 transposonThis study
    pJY034EtpB cloned in-frame with polyhistidine tag on pET-30a(+)This study
    pET-30a(+)IPTG-inducible expression plasmid; KmrNovagen
  • a Ampr, Kmr, Cmr, and Smr represent ampicillin, kanamycin, chloramphenicol, and streptomycin resistance, respectively.

  • b NEB, New England Biolabs.