TABLE 1.

Secretome proteins that make putative or confirmed contributions to L. pneumophila virulence identified by 2-DE analysis

2-DE analysisaGenInfo Identifier no.bIdentity (as defined in the genome)cGene designation ind:CharacteristicsReference(s)
OMVSSPPhiladelphia-1LensParis
+52840695IcmK (DotH)lpg0450lpl0492lpp0516Part of core transmembrane complex (type IV secretion system)77
+52841028mip; macrophage infectivity potentiator (Mip)lpg0791lpl0829lpp0855Peptidyl-prolyl isomerase domain; protein-protein interaction; promotes phospholipase C activity and transmigration through lung epithelial cells25, 78
+52841206Ecto-ATP diphosphohydrolase IIlpg0971lpl1000lpp1033Eukaryote-like; eukaryotic GDA1/CD39 NTPDase family homolog; phosphoesterase/phosphatase69
+52841570fliC; flagellinlpg1340lpl1293lpp1294Flagellar assembly; involved in evasion and spreading; type II secreted26
+52841685Phospholipase Clpg1455lpl1573lpp1411Phospholipase; plcB homolog26
+52842368LaiElpg2154lpl2082lpp2093SidE paralog; type IV secreted16
+52842717SdeD (LaiF)lpg2509lpl2431lpp2577SidE paralog; type IV secreted16
+52843033Phospholipase/lecithinase/hemolysin, lysophospholipase Alpg2837lpl2749lpp2894Phospholipase; plaC homolog10, 26
++52840696IcmE (DotG)lpg0451lpl0493lpp0517Part of core transmembrane complex (type IV secretion system)77
++52840712Zinc metalloprotease (ProA1, Msp)lpg0467lpl0508lpp0532Protease/peptidase; contributes to tissue damage in vivo; type II secreted26, 55, 66
++52840747Phosphatidylcholine-hydrolyzing phospholipaselpg0502lpl0541lpp0565Phospholipase; plcA homolog8, 26
++52840925htpB; Hsp60, 60-kDa heat shock protein HtpBlpg0688lpl0724lpp0743GroEL chaperonin family member; protein-protein interaction; involved in adherence and invasion37
++52841350Chitinase (ChiA)lpg1116lpl1121lpp1117Glycosylase; promotes persistence in the lung; type II secreted26
++52841353Major acid phosphatase (Map)lpg1119lpl1124lpp1120Eukaryote-like; phosphoesterase/phosphatase; type II secreted26
++52842435TPR repeat protein, protein-protein interactionlpg2222lpl2147lpp2174Eukaryote-like; lpnE homolog (enhC-like)26
++52842850sclB; tail fiber proteinlpg2644lpl2569lpp2697Eukaryote-like; domain homology to type VI collagen; type II secreted26, 70
++52842895IcmX (IcmY)lpg2689lpl2616lpp2743Involved in type IV secretion; required for biogenesis of the replicative organelle; type II secreted26, 53
++52843192legP; astacin proteaselpg2999lpl2927lpp3071Eukaryote-like; astacin protease; type II secreted26, 73
+52840667legY; amylaselpg0422lpl0465lpp0489Eukaryote-like; amylase73
+52840945IcmL-likelpg0708lpl0745lpp0763Putatively involved in type IV secretion77
+52841883lasB; class 4 metalloprotease (elastase)lpg1655lpl1620lpp1626ProA-like protease/peptidase
+52842236Serine metalloproteaselpg2019lpl1996lpp2001Protease/peptidase
+52842419WipClpg2206lpl2131lpp2157IcmW-interacting protein; type IV secreted16
+52842553sseJ; lysophospholipase Alpg2343lpl2264lpp2291Phospholipase; plaA homolog; type II secreted26, 33
+52842794legS1; lipid phosphoesteraselpg2588lpl2511lpp2641Eukaryote-like; signaling lipid related domain; lipid phosphoesterase73