TABLE 2.

DNA primers used in this study

Primer use and nameNucleotide sequence
Construction of mutant B. ovis PA strains
    31MUT-F5′-AGA ATA AAA CAC ATG CCC-3′
    31MUT-R5′-GCT GAA TGC GGA GAT GGT-3′
    25MUTZ-F5′-CGA CCT TAT CCT CCT GAA-3′
    25MUTZ-R5′-CCA GCA AAA CGT CGC AAA-3′
    25cdMUT-F5′-TGC GTG GTT CAG ATT TCG-3′
    25cdMUT-R5′-TTG CCG CTT CCA TCA GGT-3′
    25cMUT-R5′-AGC CGT AAC CAA CCT GAC-3′
    22MUT-F5′-GGC AAA GAA GAA GAA TAC-3′
    22MUT-R5′-CTG CTG GAA TGC CCT GAA-3′
Complementation of mutant B. ovis PA strains
    31sd5′-TGA CAG ACT TTT TCG CCG AA-3′
    31ter5′-CAT TCA GGA CAA TTC CCG CC-3′
    25A-F5′-GGA CCG CGC AAA ACG TAA-3′
    25-R5′-ACC GGA TGC CTG AAA TCC-3′
    25C-F5′-CTG TGT CCT GTT TGC TAC-3′
    25C-R5′-TAT TGG GTG AGG ATT GAC-3′
    25D-F5′-CTA CAT ACA TTC GAC CAG-3′
    25D-R5′-CTG AAG GGT AAA TGC GGC-3′
    22-F5′-TCA AGC ATG TTT CCC GCC-3′
    22-R5′-GTT TGA ATC CCG GCT GTT-3′