Table 2.

Effects of washing on CDT action

Treatmenta%bof HeLa cells in cell cycle phase:
G0/G1SG2/M
Not washed0.1 ± 0.17.4 ± 6.592.5 ± 6.5
Washed at 60 min2.6 ± 1.27.5 ± 7.189.9 ± 8.0
Washed at 40 min5.8 ± 1.910.0 ± 4.884.2 ± 6.4
Washed at 20 min12.4 ± 2.714.0 ± 4.273.6 ± 6.9
Medium control57.2 ± 5.426.8 ± 4.816.0 ± 1.8
  • a HeLa cells were treated with either recombinant CDT or EMEM (medium control). Times reflect times after addition of CDT.

  • b Values are means ± standard deviations.