Table 1.

DNA content analysis of HeLa cells treated with CDT

Time after addition (h)Additiona%bof HeLa cells in cell cycle phase:
G0/G1SG2/M
24Medium60.3 ± 2.128.6 ± 8.611.2 ± 6.7
C. jejuni81-1768.3 ± 1.427.8 ± 19.263.9 ± 18.1
C. jejuni cdtmutant62.0 ± 4.327.7 ± 6.810.3 ± 5.5
48Medium68.5 ± 3.224.8 ± 3.56.8 ± 0.4
C. jejuni81-1762.2 ± 2.90.5 ± 0.897.3 ± 2.4
C. jejuni cdtmutant64.3 ± 8.227.0 ± 2.68.6 ± 5.7
72Medium67.9 ± 4.726.0 ± 4.06.0 ± 1.1
C. jejuni81-1761.1 ± 1.80.0 ± 0.098.9 ± 1.8
C. jejuni cdtmutant62.2 ± 7.924.7 ± 2.213.2 ± 6.5
  • a Additions consisted of either EMEM (medium), a CDT-containing extract from wild-type C. jejuni81-176, or a homologous extract from the cdt mutant derivative of 81-176.

  • b Values are means ± standard deviations.