Table 1.

Sequences of oligonucleotides used in PCR amplification of SRS2, SRS3, and SRS4 fragments

OligonucleotideSequencea
SRS2ORF35′-aagctcgagTCAATAGGCAAGTGC-3′
SRS2ORF55′-ctcggatccAGAATGGCGACGCGTGC-3′
SRS255′-cgggatccCCTGAAAAGTTTACATGCCGCC-3′
SRS235′-tgctctagaTTATCCACCTGGTCTTGATCC-3′
SRS3ORF55′-tgactcgagGAAATGCGCTTACAG-3′
SRS3ORF35′-cgactcgagCTACACAAGGGAAGA-3′
SRS355′-gcgtctagaGAGAAGCAGGTAGTGTGTCCT-3′
SRS335′-acgtctagaTCAGATTGTGAGCTCTACTGT-3′
SRS455′-gcggaattcTTCGTCGGCTGTGACAACAA-3′
SRS435′-tgcggatccCGTCTGGACAGCTGAAAATT-3′
dTAP5′-ccggaattcggtacctctagat18n-3′
AP5′-tctagaggtaccgaattccgg-3′
  • a Sequences in lowercase are for cloning purposes and are not derived from Toxoplasma genes.