Table 3.

Characteristics of predicted ORFs

Potential ORF identifiedNo. of amino acidsaMol mass (kDa)bPredicated cellular locationcPredicted motifsdPredicted homologye
icmE
icmG 26929.7IM (1 TM)NoneNone
icmC 19420.3IM (4 TM)NoneNone
icmD 13213.5IM (1 TM)NoneNone
icmJ 21224.2IM (1 TM)NoneNone
icmB 1,009112.1IM (1 TM)ATP/GTP binding siteNone
tphA 41846.4IM (12 TM)Sugar transport proteinProP
icmF 973110.7IM (2 TM)ATP/GTP binding siteNone
  • a The number of amino acids encoded by each complete ORF is reported. icmE is partial on pMW100; therefore the total number of amino acids is not reported.

  • b The predicted molecular mass of each complete ORF is shown. The molecular mass of the partial icmE cannot be determined.

  • c The location of each ORF was predicted by the Psort program (26). IM designates an inner membrane location. The number of transmembrane domains (TM) as determined by the Psort program is shown in parentheses.

  • d Motif searches were performed with the GCG motif search program.

  • e Homology searches were performed at the amino acid and nucleotide levels by using the TFASTA, BLASTP, and BLASTX (18) programs.