Table 1.

Primer and probe sequences for PCR and ELOCA

Primer or probeSequence(s)aPCR product size (bp)
Primers
 IL-1β5′ CTC CGG GAC TCA CAG CAA AAA 3′327
5′ GGG GAA CTG GGC AGA CTC A 3′
 IL-85′ TTG GCA GCC TTC CTG ATT TCT 3′220
5′ TTT CCT TGG GGT CCA GAC AGA 3′
 ENA-785′ TGT TGG TGC TGC TGC TG 3′212
5′ ACA AAT TTC CTT CCC GTT CTT 3′
 iNOS5′ ATG GAA CAT CCC AAA TAC GA 3′453
5′ CAG CAT CTC CTG GTG AAA CA 3′
 GAPDH5′ GGT GAA GTT CGG AGT CAA CGG A 3′240
5′ GAG GGA TCT CGC TCC TGG AAG A 3′
Probesb
 IL-1β5′ CCA CCT CCA GGG ACA 3′
 IL-85′ TCA TTG AGA GTG GAC C 3′
 ENA-785′ ACG CAA CGC AGC TCT 3′
 iNOS 5′ TTC TCC TGC CCA CTT C 3′
 GAPDH5′ GCC TTG ACG GTG CCA T 3′
  • a For each primer pair, the sense primer is given above the antisense primer.

  • b Labelled directly with DIG via a six-carbon spacer.