Table 1.

Bacterial strains used in this study

StrainGenotype and featuresReference or source
L. pneumophila
 JR32NaCl-sensitive isolate of AM511 28
 LELA1205JR32icmD1205::Tn903dIIlacZ, DNA hybridization group II 28
 LELA1506JR32icmB1506::Tn903dIIlacZ, DNA hybridization group II 28
 LELA1984JR32icmE1984::Tn903dIIlacZ, DNA hybridization group II 28
 LELA1996JR32icmB1996::Tn903dIIlacZ, DNA hybridization group II 28
 LELA2517JR32icmC2517::Tn903dIIlacZ, DNA hybridization group II 28
 LELA3244JR32icmD3244::Tn903dIIlacZ, DNA hybridization group II 28
 LELA3379JR32icmB3379::Tn903dIIlacZ, DNA hybridization group II 28
 LELA3393JR32icmB3393::Tn903dIIlacZ, DNA hybridization group II 28
 LELA3563JR32icmB3563::Tn903dIIlacZ, DNA hybridization group II 28
 LELA3896JR32icmB3896::Tn903dIIlacZ, DNA hybridization group II 28
 LELA1223JR32icmB1223::Tn903dIIlacZ, DNA hybridization group IV 28
 LELA1566JR32icmB1566::Tn903dIIlacZ, DNA hybridization group IV 28
 LELA3150JR32icmB3150::Tn903dIIlacZ, DNA hybridization group IV 28
 LELA3323JR32icmB3323::Tn903dIIlacZ, DNA hybridization group IV 28
 LELA1012JR32icmB1012::Tn903dIIlacZ, DNA hybridization group VI 28
 LELA2947JR32icmB2947::Tn903dIIlacZ, DNA hybridization group VI 28
 LELA1275JR32icmF1275::Tn903dIIlacZ, DNA hybridization group XVII 28
 LELA1718JR32icmF1718::Tn903dIIlacZ, DNA hybridization group XVII 28
 MW635JR32icmG::Kmr This study
 MW645JR32 icmC::Kmr This study
 MW656JR32icmJ::Kmr This study
 MW627JR32 tphA::Kmr This study
 Philadelphia-1Virulent, serogroup 1 16
E. coli
 DH5αF endA1 hsdRI7(r k m+ k) supE44 thi-1 λ recA1 relA1 Δ(argF-lacZYA) U169 φ80dlacZΔM15 deoR gyrA96Nalr 33
 LE392F e14 (McrA)hsdR514 (r km+ k) supE44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55 3
 LW211LE392 with an integrated RP4 (Δbla tetA::Mu) transferred by P1 transduction from SM10; Tra+ KmrApr Tcs 28
 MW67DH5α containing a L. pneumophilaPhiladelphia-1 chromosomal DNA library consisting of partialEcoRI fragments (10–20 kb) in pMMB207This study